Question 1

Media that changes color as a result of the biochemical activity of growing bacteria

Accepted Answer

A)  usually contain a pH indicator in the media.
B)  usually contain blood.
C)  usually require the addition of various reagents before the color is evident.
D)  is due to a breakdown of a colorless reagent.E) B) and D)
F) A) and B)

Question 2

Which technique(s)  is/are used to help identify and classify bacteria?

Accepted Answer

A)  microscopic examination
B)  culture characteristics
C)  biochemical tests
D)  nucleic acid analysis
E)  All of the choices are correct.F) A) and B)
G) B) and C)

Question 3

To study the phylogeny of eukaryotes

Accepted Answer

A)  16S rRNA is used.
B)  34S rRNA is used.
C)  80S rRNA is used.
D)  18S rRNA is used.E) A) and B)
F) A) and C)

Question 4

All known species of bacteria are described in Bergey's Manual of Systematic Bacteriology.

Accepted Answer

A) True 
 B)False

Question 5

The three domain systems consist of Archaea, Bacteria, Eucarya.

Accepted Answer

A) True 
 B)False

Question 6

The reference for taxonomic descriptions of bacteria is

Accepted Answer

A)  Gray's Anatomy.
B)  Websters Manual of Taxonomic Bacteriology.
C)  Bergey's Manual of Systematic Bacteriology.
D)  Bacteriology.E) A) and B)
F) B) and C)

Question 7

Gram-positive encapsulated diplococci found in sputum is indicative of

Accepted Answer

A)  E.coli.
B)  Pseudomonas.
C)  Streptococcus pneumoniae.
D)  Neisseria gonorrhea.E) A) and B)
F) B) and D)

Question 8

Phylogenetic relationships between prokaryotes are most accurately determined using

Accepted Answer

A)  phenotypic characterizations.
B)  genotypic information.
C)  the fossil record.
D)  carbon dating.
E)  phenotypic characterizations AND genotypic information.F) A) and D)
G) B) and C)

Question 9

The ticks of a genetic clock are measured by the

Accepted Answer

A)  number of random mutations.
B)  number of dead cells.
C)  amount of RNA.
D)  amount of DNA.E) A) and D)
F) None of the above

Question 10

A sample must contain many microorganisms in order to see any using microscopy techniques. Why?

Accepted Answer

A)  The area on a microscope slide is very large compared to the relative size of microbes, and you take a very small amount of your sample to place on the slide surface.Therefore, you must have a large number of microbes initially in the culture to increase the chances that you'll come across one or more when looking at the slide surface through the magnifying lenses of the microscope.
B)  Bacteria are very small, of course.If you don't have a LOT of them, how are you going to see them?
C)  This is false-microscopy allows us to magnify a specimen to the point where we would be able to visualize even just a few microbes on the surface of the slide.As such, even if there are only a few microbes in a sample, we could easily visualize them with a microscope.
D)  Microscopes only magnify what's on the slide.If you don't have a lot of microbes on the slide surface, you can't magnify the specimen enough to see them as individual cells.E) B) and D)
F) A) and B)

Question 11

In higher organisms, successful mating can occur between members of the same

Accepted Answer

A)  genus.
B)  species.
C)  class.
D)  order.E) A) and D)
F) B) and C) B

Question 12

The point at which two organisms diverged from a common ancestor

Accepted Answer

A)  can be determined by comparing the nucleic acid sequences.
B)  depends on translational control.
C)  depends on metabolism.
D)  is determined on MacConkeys media.E) A) and B)
F) C) and D)

Question 13

The relatedness of organisms determined by counting common characteristics is called

Accepted Answer

A)  evolutionary taxonomy.
B)  amino acid sequences.
C)  DNA sequences.
D)  numerical taxonomy.
E)  suggests the organisms are very closely related at the species level AND means the GC content is 45%.F) None of the above
G) C) and E)

Question 14

The three domain classification scheme uses

Accepted Answer

A)  order, phylum, class.
B)  plants, animals, bacteria.
C)  protista, prokaryotae, fungaea.
D)  Archaea, Bacteria, Eucarya.E) None of the above
F) All of the above

Question 15

When DNA probes are used to identify bacterial DNA similarities by hybridization, the probe DNA is heated and the template DNA is treated to separate the 2 strands. Why would the probe DNA be heated?

Accepted Answer

A)  This is the only way to properly label the probe DNA.
B)  The probe DNA is single-stranded, but it may have folded back on itself and formed portions that are double-stranded.Heating it up breaks any possible hydrogen bonds that may have formed, returning it to a single-stranded state prior to hybridization.
C)  Heating it up activates the tag on the probe DNA before it hybridizes to the bacterial template DNA.
D)  DNA hybridization can only take place at high temperatures, so all the DNA must be heated up prior to hybridization.E) None of the above
F) A) and D) B

Question 16

A selective growth medium

Accepted Answer

A)  allows only certain bacteria to grow.
B)  allows all bacteria to grow.
C)  allows no bacteria to grow.
D)  accentuates differences between the growing bacteria.E) None of the above
F) B) and C)

Question 17

The name Lactococcus (Streptococcus)  lactis

Accepted Answer

A)  indicates that the subgenus is Streptococcus.
B)  indicates that the family is Lactococcus.
C)  indicates that Streptococcus is the old genus name.
D)  indicates that Lactococcus is the old class name.E) A) and B)
F) None of the above C

Question 18

The more closely related two organisms are,

Accepted Answer

A)  the less they look alike.
B)  the more similar the nucleic acid sequence.
C)  the less similar the nucleic acid sequence.
D)  the more they are phenotypically similar.
E)  the more similar the nucleic acid sequence AND the more they are phenotypically similar.F) A) and B)
G) A) and C)

Question 19

Intracellular Gram-negative diplococci found in a urethral sample from a male is indicative of

Accepted Answer

A)  E.coli.
B)  Pseudomonas.
C)  Streptococcus pneumoniae.
D)  Neisseria gonorrhea.E) A) and C)
F) None of the above

Question 20

Explain the difference between a biotype and a serotype.

Accepted Answer

A)  A biotype is the living type of microorganism identified, while the serotype is an individual's type of blood serum.
B)  A biotype is the type of biological environment a microbe will grow in, while the serotype is the type of blood serum that the microbe can grow in.
C)  A biotype is the particular phenotypic traits and characteristic structures of a biological microorganism, while the serotype is the list of molecules the microbe is able to make and secrete into the serum of an infected individual.
D)  A biotype is the biochemical profile of a particular microorganism, while the serotype is the difference in its surface structures/antigens that will lead to separate immune responses from an organism (characterized by presence of different antibodies in the individual's serum) .E) B) and C)
F) A) and D)

Exam 10: Identification and Classification of Prokaryotes

Media that changes color as a result of the biochemical activity of growing bacteria

Correct AnswerverifiedShow Answer

Which technique(s) is/are used to help identify and classify bacteria?

Correct AnswerverifiedShow Answer

To study the phylogeny of eukaryotes

Correct AnswerverifiedShow Answer

All known species of bacteria are described in Bergey's Manual of Systematic Bacteriology.

Correct AnswerverifiedShow Answer

The three domain systems consist of Archaea, Bacteria, Eucarya.

Correct AnswerverifiedShow Answer

The reference for taxonomic descriptions of bacteria is

Correct AnswerverifiedShow Answer

Gram-positive encapsulated diplococci found in sputum is indicative of

Correct AnswerverifiedShow Answer

Phylogenetic relationships between prokaryotes are most accurately determined using

Correct AnswerverifiedShow Answer

The ticks of a genetic clock are measured by the

Correct AnswerverifiedShow Answer

A sample must contain many microorganisms in order to see any using microscopy techniques. Why?

Correct AnswerverifiedShow Answer

In higher organisms, successful mating can occur between members of the same

Correct AnswerverifiedB

The point at which two organisms diverged from a common ancestor

Correct AnswerverifiedShow Answer

The relatedness of organisms determined by counting common characteristics is called

Correct AnswerverifiedShow Answer

The three domain classification scheme uses

Correct AnswerverifiedShow Answer

When DNA probes are used to identify bacterial DNA similarities by hybridization, the probe DNA is heated and the template DNA is treated to separate the 2 strands. Why would the probe DNA be heated?

Correct AnswerverifiedB

A selective growth medium

Correct AnswerverifiedShow Answer

The name Lactococcus (Streptococcus) lactis

Correct AnswerverifiedC

The more closely related two organisms are,

Correct AnswerverifiedShow Answer

Intracellular Gram-negative diplococci found in a urethral sample from a male is indicative of

Correct AnswerverifiedShow Answer

Explain the difference between a biotype and a serotype.

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
B

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
B

Correct Answer
verified

Correct Answer
verified
C

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified